Journal: The Turkish Journal of Gastroenterology

Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis

doi: 10.5152/tjg.2021.211106

Figure Lengend Snippet: Study Characteristics by Immunohistochemistry Methods

Article Snippet: - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS Hu 201642 Tissue Microarray Anti-Sp1 (CST, Beverly, MA, USA) dilution 1 : 1000 NS - Sp1 (+) = score > 6 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Jiang 20089 Whole section Mouse monoclonal anti-Sp1 at 2 μg/mL Biotin-free polymer-based - Sp1 (+) = >20% cells stained - Negative Control = sections stained with nonimmune mouse IgG (vector) at a concentration of 2 μg/mL Weichert 200743 Whole section Monoclonal RelA-antibody (sc-8008, Santa Cruz Biotechnology, Santa Cruz, CA, USA) dilution 1 : 250 Streptavidin–biotin–peroxidase system Nuclear NF-κB (+) = stained nuclei of tumor cells representing the expression of RelA NS Yang 201344 Whole section Anti-NF-κBp65 (sc-109, Santa Cruz Biotechnology) dilution 1 : 50 Avidin–biotin–peroxidase system Nuclear NF-κB (+) = > 10% of tumor cells had positively stained nuclei NS Open in a separate window NS, not specified; PDAC, pancreatic ductal adenocarcinoma; COX-2, Cyclooxygenase-2; Sp1, specificity protein 1; NF-κB, nuclear factor-kappaB.

Techniques: Immunohistochemistry, Polymer, Negative Control, Staining, Protein Concentration, Avidin-Biotin Assay, Positive Control, Saline, Microarray, Plasmid Preparation, Concentration Assay, Expressing

Journal: The Turkish Journal of Gastroenterology

Article Title: Prognostic Value of COX-2, NF-κB, and Sp1 Tissue Expressions in Pancreatic Ductal Adenocarcinoma: A Systematic Review and Meta-analysis

doi: 10.5152/tjg.2021.211106

Figure Lengend Snippet: Study Characteristics by Immunohistochemistry Methods

Article Snippet: - 1 = < 10% cells stained, 2 = 10-50% and 3 > 50% Schmid 201340 Whole section Mouse monoclonal anti-COX-2 antibody (clone CX220; Cayman Chemical, Ann Arbor, Mich) dilution 1 : 200 Streptavidin–biotin–peroxidase system - COX-2 (+) = detectable staining - Negative Control: incubating the sections without primary antibody - Internal positive control: immunoreactivity of the pancreatic islets Tonini 200541 Whole section Goat polyclonal anti-COX-2 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), dilution not specified Streptavidin–biotin–peroxidase system - COX-2 (+) = score ≥+1 (positive staining) - Negative control: sections with rabbit or goat preimmune serum Hang 201629 Tissue Microarray Anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS - COX-2 or Sp1 (+) = score ≥ 4 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Anti-Sp1 (Cell Signaling Technology, Beverly, MA, USA), dilution not specified NS Hu 201642 Tissue Microarray Anti-Sp1 (CST, Beverly, MA, USA) dilution 1 : 1000 NS - Sp1 (+) = score > 6 NS - Score = staining intensity (0-3) x % of cells stained (1-4) Jiang 20089 Whole section Mouse monoclonal anti-Sp1 at 2 μg/mL Biotin-free polymer-based - Sp1 (+) = >20% cells stained - Negative Control = sections stained with nonimmune mouse IgG (vector) at a concentration of 2 μg/mL Weichert 200743 Whole section Monoclonal RelA-antibody (sc-8008, Santa Cruz Biotechnology, Santa Cruz, CA, USA) dilution 1 : 250 Streptavidin–biotin–peroxidase system Nuclear NF-κB (+) = stained nuclei of tumor cells representing the expression of RelA NS Yang 201344 Whole section Anti-NF-κBp65 (sc-109, Santa Cruz Biotechnology) dilution 1 : 50 Avidin–biotin–peroxidase system Nuclear NF-κB (+) = > 10% of tumor cells had positively stained nuclei NS Open in a separate window NS, not specified; PDAC, pancreatic ductal adenocarcinoma; COX-2, Cyclooxygenase-2; Sp1, specificity protein 1; NF-κB, nuclear factor-kappaB.

Techniques: Immunohistochemistry, Polymer, Negative Control, Staining, Protein Concentration, Avidin-Biotin Assay, Positive Control, Saline, Microarray, Plasmid Preparation, Concentration Assay, Expressing